01765nas a2200349 4500000000100000008004100001260001300042653002700055653002200082653002100104653001100125653002300136653002800159653001200187653002600199653002500225653000900250653002600259653001800285653003400303653005700337100001300394700001500407700001500422700001500437700001200452245009000464300001000554490000700564520083000571022001401401 1987 d c1987 Sep10aAntibodies, Monoclonal10aAntigens, Surface10aErythema Nodosum10aHumans10aImmunity, Cellular10aImmunoenzyme Techniques10aleprosy10aLymphocyte Activation10aMycobacterium leprae10aSkin10aStaining and Labeling10aT-Lymphocytes10aT-Lymphocytes, Helper-Inducer10aTumor Necrosis Factor Receptor Superfamily, Member 71 aShen J Y1 aHofman F M1 aGunter J R1 aModlin R L1 aRea T H00aIn situ identification of activated Ta1+ T lymphocytes in human leprosy skin lesions. a494-80 v553 a
Using a monoclonal antibody, anti-Ta1, that identifies antigen-activated T lymphocytes in vitro, we sought to identify activated T lymphocytes in leprosy skin lesions. Greater numbers of Ta1 positive T lymphocytes were observed in tuberculoid leprosy, lepromin skin tests, and reversal reactions as compared with lepromatous leprosy or erythema nodosum leprosum (ENL) (p less than 0.001). With a double-staining technique, we found that the majority of these activated T lymphocytes were of the helper/inducer phenotype. No differences of Ta1 positive lymphocytes were observed in the peripheral blood. The defective cell-mediated immune response in lepromatous and ENL patients correlates with, and may be related to the failure of T-helper/inducer activation or proliferation in the presence of Mycobacterium leprae.
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