02060nas a2200397   4500000000100000008004100001260001200042653003100054653001400085653002500099653002400124653002500148653001700173100001000190700001100200700001000211700000900221700001200230700001000242700001000252700000900262700000900271700001000280700001100290700001100301700000900312700001100321700001000332700001000342245013900352856007700491300001400568490000700582520105900589022001401648       2022                            d  c01/202210aMycobacterium tuberculosis10adiagnosis10aDisease transmission10adroplet digital PCR10adrug resistance test10aTuberculosis1 aFan Y1 aChen J1 aLiu M1 aXu X1 aZhang Y1 aYue P1 aCao W1 aJi Z1 aSu X1 aWen S1 aKong J1 aZhou G1 aLi B1 aDong Y1 aLiu A1 aBao F00aApplication of Droplet Digital PCR to Detection of Mycobacterium tuberculosis and Mycobacterium leprae Infections: A Narrative Review.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934166/pdf/idr-15-1067.pdf  a1067-10760 v153 a<p>Tuberculosis (TB) is a chronic infectious disease caused by infection, which has seriously endangered human health for many years. With the emergence of multidrug-resistant and extensively drug-resistant , the prevention and treatment of TB has become a pressing need. Early diagnosis, drug resistance monitoring, and control of disease transmission are critical aspects in the prevention and treatment of TB. However, the currently available diagnostic technologies and drug sensitivity tests are time consuming, and thus, it is difficult to achieve the goal of early diagnosis and detection drug sensitivity, which results in limited control of disease transmission. The development of molecular testing technology has gradually achieved the vision of rapid and accurate diagnosis of TB. Droplet digital PCR (ddPCR) is an excellent nucleic acid quantification method with high sensitivity and no need for a calibration curve. Herein, we review the application of ddPCR in TB diagnosis and drug resistance detection and transmission monitoring.</p>
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