02528nas a2200289   4500000000100000008004100001260001200042653002500054653001500079653001400094653001200108653002700120100001100147700001000158700001000168700001100178700001100189700001000200700000900210700001200219245014900231856008100380300001100461490000700472520174500479022001402224       2021                            d  c01/202110aMycobacterium leprae10aBiomarkers10adiagnosis10aleprosy10atranscriptome analysis1 aYuan Y1 aLiu J1 aYou Y1 aChen X1 aYuan L1 aWen Y1 aLi H1 aZhang Y00aTranscriptomic Analysis of Stimulated Response in Peripheral Blood Mononuclear Cells Reveal Potential Biomarkers for Early Diagnosis of Leprosy.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724050/pdf/fcimb-11-714396.pdf  a7143960 v113 a<p>We aimed to identify an unique host transcriptional signature in peripheral blood mononuclear cells (PBMCs) in response to antigens to distinguish between patients with leprosy and non-leprosy controls for early diagnosis of the disease. Sixteen individuals were enrolled in the discovery cohort [eight patients with leprosy, comprising four multibacillary (MB) and four paucibacillary (PB); and eight non-leprosy controls, comprising four healthy house contacts (HHCs) and four endemic controls (ECs)]. The differences in the transcriptome response of PBMCs to sonicate antigen were evaluated between leprosy patients and non-leprosy controls, and 12 differentially expressed genes (, , , , , , , , , , and ) were identified. The accuracy of the 12 differentially expressed genes was further validated for the diagnosis of leprosy using real-time quantitative PCR in 82 individuals (13 MB, 10 PB, 37 HHCs, and 22 ECs) in the validation cohort. We found that a 5 gene signature set , , , and had a suitable performance in discriminating leprosy from ECs. In addition, elevated expression of , , and was associated with MB diagnosis compared with ECs, whereas increased expression of , , and was found to be useful biomarkers for PB diagnosis from ECs. Moreover, we found decreased expression of among leprosy patients could distinguish leprosy from HHCs, whereas higher expression of among MB than PB could distinguish different leprosy patients. In conclusion, among the 12 candidate host genes identified, a three gene signature , and showed the best performance in distinguishing leprosy patients from healthy controls. These findings may have implications for developing a rapid blood-based test for early diagnosis of leprosy.</p>
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