00975nas a2200217 4500000000100000008004100001260001200042653002700054653003300081653001100114653001700125653003000142653003000172100001400202700001300216700001200229700001200241245015400253520033600407022001400743 2021 d c10/202110a Mycobacterium leprae 10a Mycobacterium lepromatosis 10a rpoT 10aCo-infection10aMolecular diagnostic test10apolymerase chain reaction1 aDwivedi P1 aSharma M1 aPatel P1 aSingh P00aSimultaneous detection and differentiation between Mycobacterium leprae and Mycobacterium lepromatosis using novel polymerase chain reaction primers.3 a

We have developed a polymerase chain reaction-based method to detect and distinguish Mycobacterium leprae and Mycobacterium lepromatosis using a single set of primers based on a 45-bp difference in the amplicon size of their rpoT gene. This method can also help in detecting the cases of co-infection in a single experiment.

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