01595nas a2200169 4500000000100000008004100001260002400042653002200066100001400088700001500102700002000117245007400137856007900211490000700290520110300297022002501400 2021 d bFapUNIFESP (SciELO)10aMultidisciplinary1 aAfonso AS1 aMadurro JM1 aBrito-Madurro A00aElectrochemical DNA Biosensor for Mycobacterium leprae Identification uhttps://www.scielo.br/j/babt/a/WMdnLvpNLVWbbLRPR6HgBTD/?format=pdf&lang=en0 v643 aNowadays, a prompt and reliable diagnosis is one of the most critical measures for leprosy control. The current diagnostic is based on clinical exams by a health care professional, and it may not recognize early signs of the disease. Therefore, other leprosy diagnosis methods are needed that are sensitive, disease-specific, and easy to deliver to the end-user. This study describes the construction of an electrochemical DNA biosensor to detect PCR products of Mycobacterium leprae using methylene blue as an indicator of the hybridization. The capture probe was immobilized on the graphite electrode modified with poly(4-aminophenol). The electrode surface was morphologically characterized by atomic force microscopy. Linear voltammetry was used to monitor the concentration of methylene blue on the DNA biosensor, which indicated a limit detection of 1 x 10-10 mol/L. The biosensor showed selective when placed to hybridize with a non-complementary sequence. This study suggests that the electrochemical DNA biosensor developed is promising for detecting DNA of Mycobacterium leprae. a1678-4324, 1516-8913