02323nas a2200433   4500000000100000008004100001260001300042653001000055653002400065653001600089653002800105653001200133653003100145653003800176653001100214653001100225653002500236653000900261653001600270653002800286653002500314653001600339100001600355700001500371700001600386700001600402700001500418700001300433700001700446700001200463700001600475245019600491856005900687300000900746490000700755050003200762520108100794022001401875       1989                            d  c1989 Mar10aAdult10aAntigens, Bacterial10aClofazimine10aColony Count, Microbial10aDapsone10aDrug Resistance, Microbial10aEnzyme-Linked Immunosorbent Assay10aFemale10aHumans10aLeprosy, lepromatous10aMale10aMiddle Aged10aMonitoring, Physiologic10aMycobacterium leprae10aPhilippines1 aDouglas J T1 aHirsch D S1 aFajardo T T1 aCellona R V1 aAbalos R M1 aCruz E C1 aMadarang M G1 aWit M Y1 aKlatser P R00aEvaluation of Mycobacterium leprae antigens in the serological monitoring of a clofazimine-based chemotherapeutic study of dapsone resistant lepromatous leprosy patients in Cebu, Philippines.  uhttp://leprev.ilsl.br/pdfs/1989/v60n1/pdf/v60n1a02.pdf  a8-190 v60  aInfolep Library - available3 a<p>Thirty-one dapsone resistant lepromatous leprosy patients receiving clofazimine based therapy were serologically monitored throughout their 5-year period of treatment. Sequentially collected sera were used to examine 4 Mycobacterium leprae antigens to evaluate their usefulness in ELISA's for monitoring the progress of their therapy. The ELISA results were compared with decline in bacterial load over the treatment period and with duration of treatment. In addition the ELISA's were compared with each other. The ELISA's based on the measurement of IgM antibodies to the two neoglycoproteins (NDO and NTO) representing the phenolic glycolipid antigen of M. leprae were found to be the most effective with regard to monitoring treatment. A whole M. leprae based ELISA was less efficient in monitoring treatment because it failed to measure antibodies in 5 out of 31 patients. The ELISA-inhibition test based on the detection of antibodies to a species-specific epitope on the 36 K antigen of M. leprae was less suitable because of persistent reactivity during therapy.</p>  a0305-7518