01742nas a2200193   4500000000100000008004100001260002300042100001900065700002000084700002400104700001800128700001500146245009000161856010400251300001200355490000600367520116100373022001401534       2019                            d  bBlood and Genomics1 aMercado Ceja S1 aRangel Gamboa L1 aElisa Vega Memije M1 aOlivo Díaz A1 aGranados J00aHLA class II alleles with susceptibility of leprosy in the Mexican Mestizo population  uhttp://www.apjbg.com/ch/reader/create_pdf.aspx?file_no=2019117&flag=1&journal_id=apjbg&year_id=2019  a133-1370 v33 aLeprosy is a chronic, infectious disease, caused by Mycobacterium leprae, Mycobacterium lepromatosis or both, which affects the peripheral nervous system and the skin. Activation of cellular immunity in infected individuals depends on antigen recognition, which involves relevant HLA-Class II alleles. Therefore, the objective of this study was to determine HLA-Class II allele frequencies (HLA-DRB1and-DQB1)in Mexican Mestizo leprosy patients and compare themwith healthy controls, in order to define their role in the genetic susceptibility to this infection.The genomic DNA of each participant was obtained from peripheral blood, using the salting-out method. PCR amplification and hybridization ofHLA-class II alleleswas made by PCR-SSO. The results showed that frequencies of HLA-DRB1*15(Pc =0.003, OR=3.3 95%CI=1.53-7.33), HLA-DQB1*05(Pc =0.00003, OR=6.03 95%CI=2.49-14.61) and HLA-DQB1*06 (Pc =0.007, OR=2.89, 95%CI=1.38-6.04)were significantly higher among leprosypatients than those of healthy controls. The study suggests that HLA-DRB1*15, HLA-DQB1*05, and HLA-DQB1*06 are associated with leprosy susceptibility in the Mexican Mestizo population.  a2519-7916