01709nas a2200265   4500000000100000008004100001260001700042653002400059653002000083653001600103653001100119653002800130653002100158653001200179653002500191100001600216700001300232700001500245245014300260300000900403490000700412050003200419520097800451022001401429       1990                            d  c1990 Jan-Mar10aAntigens, Bacterial10aGlycoconjugates10aGlycolipids10aHumans10aImmunoenzyme Techniques10aImmunoglobulin M10aleprosy10aMycobacterium leprae1 aMoudgil K D1 aMishra R1 aTalwar G P00aComparative evaluation of enzyme immunoassays based on synthetic glycoconjugates and phenolic glycolipid-I for immunodiagnosis of leprosy.  a60-50 v62  aInfolep Library - available3 a<p>Enzyme immunoassays (EIAs) based on synthetic glycoconjugates containing the terminal monosaccharide (M-BGG) or disaccharide (ND-BSA) residue of the trisaccharide component of phenolic glycolipid-I (PGL-I), for immunodiagnosis of leprosy are described. The results of the assays were compared with that of the EIA using PGL-I. All the three assays were highly specific for leprosy. The per cent positivity of active lepromatous leprosy (LL) patients with M-BGG was 78.05 in comparison to 85.36 with ND-BSA and 82.11 with PGL-I. Similarly, the positivity of tuberculoid (TT) leprosy patients in M-BGG assay was lower than that in EIAs using ND-BSA or PGL-I. However, the difference in the positivity of individual category of leprosy patients in the three EIAs was not statistically significant. The correlation between absorbance values of leprosy sera in EIAs based on M-BGG and PGL-I, as well as that in assays using ND-BSA and PGL-I was statistically significant.</p>  a0254-9395