01674nas a2200301   4500000000100000008004100001260001700042653001200059653001800071653001100089653000900100653001100109653001200120653000900132653000900141653001800150653001300168100002000181700001700201700001000218700001600228245014700244300001100391490000700402050003200409520091700441022001401358       1990                            d  c1990 Apr-Jun10aAnimals10aCulture Media10aFemale10aFoot10aHumans10aleprosy10aMale10aMice10aMycobacterium10aNocardia1 aChakrabarty A N1 aDastidar S G1 aDas S1 aChandra A K00aCultivation in vitro of acid-fast nocardioform chemoautotrophic bacteria from mouse foot-pads infected with human strains of leprosy bacillus.  a169-790 v62  aInfolep Library - available3 a<p>Four acid-fast nocardioform bacteria could be isolated and cultivated as pure cultures in vitro from mouse foot-pads (MFP), which were infected with serially passaged strains of human leprosy bacillus; the liquid mineral medium, such as paraffin urea minimal (PUM), paraffin gelatin minimal (PGM), gelatin minimal (GM), and GM agar (GMA) slants containing only simple sources of C and N were used, just like the human and the armadillo isolates of these organisms reported earlier. Morphologically, metabolically and enzymologically, these were closely related to the previous ones and were also chemoautotrophic in nature. Serologically there appears to be a heterogenicity in these isolates, i.e., some of them showing higher affinity to nocardioforms while others showing significant binding to several mycobacteria. Normal (uninfected) mouse foot-pad harvests were not found to harbour such organisms.</p>  a0254-9395