02534nas a2200253 4500000000100000008004100001260002300042653001600065653001900081100001400100700001700114700001300131700001500144700001300159700001300172700001900185700001500204245010400219856016900323300001200492490000900504520175300513022001402266 2020 d bInforma UK Limited 10 aDermatology 10 aFlow Cytometry 1 aFarag AGA 1 aEl Askary SA 1 aFathy WM 1 aElbassal F 1 aAzzam AA 1 aTayel NR 1 aAbdul Karim SS 1 aShehata WA 00 aRelationship Between sCD163 and mCD163 and Their Implication in the Detection and Typing of Leprosy uhttps://www.dovepress.com/front_end/cr_data/cache/pdf/download_1592480001_5eeb510139d78/ccid-240420-relationship-between-scd163-and-mcd163-and-their-implication.pdf a379-389 0 v2020 3 aBackground: Leprosy is a chronic contagious disease caused by Mycobacterium lepraea. CD163 is a monocyte trans-membrane glycoprotein receptor (mCD163) that sheds from the cell surface and circulates as a soluble (serum) form (sCD163). Changes in the mCD163 and sCD163 levels could mirror the categorization of inflammatory procedure, demonstrating a possible use of CD163 as a diagnostic indicator of inflammation.
Objective: To investigate the possible role of CD163 (sCD163 and mCD163) in leprosy pathogenesis and to assess whether CD163 is a helpful inflammatory marker of leprosy development and typing.
Patients and Methods: This case control study included 70 leprosy patients and 30 healthy controls. Leprosy patients were classified according to the Madrid criteria (1953) into: tuberculoid leprosy (TT), border-line leprosy (BL), and lepromatous leprosy (LL). For all participants, complete blood count (CBC), serum CD163 using ELISA and monocytes positive for CD163 using flow cytometry were done.
Results: Leprosy patients had significantly low WBCs and platelet counts (p< 0.001) and had significantly higher sCD163 (p=0.025) and mCD163 (p=0.042) that were highest in LL followed by BL, then TT patients (p< 0.001). There was a significant positive correlation between mCD163 and sCD163 levels in leprosy patients (r=0.896, p< 0.001). ROC analysis revealed a significant role of serum sCD163 and of mCD163 positive monocytes in the detection (p< 0.001) and typing of leprosy (p=0.002 and p< 0.001, respectively).
Conclusion: Both sCD163 and mCD163 positive monocytes may have an active role in leprosy pathogenesis. They could be potential biomarkers for leprosy detection and typing. a1178-7015