02855nas a2200265   4500000000100000008004100001260001200042653001700054653002500071653003500096653002500131100001000156700001200166700001700178700001200195700001100207700001100218700001400229245011300243856006500356300001200421490000700433520213500440022001402575       2020                            d  c05/202010aEpidemiology10aMycobacterium leprae10aBorderline lepromatous leprosy10aLeprosy, lepromatous1 aDas M1 aDiana D1 aWedderburn A1 aRajan L1 aRao SP1 aHoro I1 aVedithi S00aMolecular epidemiology and transmission dynamics of leprosy among multicase families and case-contact pairs.  uhttps://www.ijidonline.com/article/S1201-9712(20)30278-2/pdf  a172-1790 v963 a<p><b>BACKGROUND: </b>Human-to-human transmission of Mycobacterium leprae among household contacts of active leprosy cases is significant, and surveillance of household contacts is vital to interrupting the transmission chain for this disease. This study was conducted to identify similarities in M. leprae strains, based on genomic single nucleotide polymorphisms (SNPs), among cases and their household contacts and in multicase families in order to decipher possible associations, transmission links, various clinical conditions of index cases that enhance person-to-person transmission, and timelines for transmission patterns.</p><p><b>METHODS: </b>PCR for M. leprae DNA detection (amplification of the Rlep gene) and SNP subtyping of M. leprae strains was performed for 61 index cases and one of their household contacts. Additionally, we studied six families with multiple cases of leprosy, to understand timelines of infectivity and its relation to severity of the disease in the index cases.</p><p><b>RESULTS: </b>Index cases with lepromatous (LL) and borderline lepromatous (BL) leprosy, together with a positive bacteriological index (BI) for M. leprae, result in a higher percentage of their contacts subclinically infected with M. leprae, with odds ratios (OR) of 6.6 (95% confidence interval (CI) 1.6-27.6) for BL and LL, and 7.07 (CI 1.41-35.41) for BI-positive index cases. 75% of the case-contact pairs had a similar SNP subtype of M. leprae. The timeline of infection in multicase families revealed that contacts were infected during the BI-positive period of the index case.</p><p><b>CONCLUSION: </b>Using molecular methods, we determined that positivity for M. leprae DNA in contacts of index leprosy cases was attributed to clinical characteristics of leprosy in the index cases. LL and BL forms of leprosy, together with positive BI, contributed to dissemination of infection to household contacts. In conclusion, we found a relationship between SNP subtypes within index case-contact pairs. This method can help decipher the transmission patterns and identify individuals at risk of contracting leprosy.</p>  a1878-3511