02039nas a2200373   4500000000100000008004100001260001300042653002600055653002400081653002800105653002400133653003800157653001600195653001100211653001600222653002500238653001200263653002500275653003000300653003100330653002600361100001100387700001100398700001200409700001100421700001000432700001200442245008600454856004100540300001100581490000700592520105200599022001401651       1990                            d  c1990 Jun10aAntibodies, Bacterial10aAntigens, Bacterial10aChi-Square Distribution10aDouble-Blind Method10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aImmune Sera10aLatex Fixation Tests10aleprosy10aMycobacterium leprae10aPredictive Value of Tests10aReproducibility of Results10aSerum Albumin, Bovine1 aWu Q X1 aYe G Y1 aYin Y P1 aLi X Y1 aLiu Q1 aWei W H00aRapid serodiagnosis for leprosy--a preliminary study on latex agglutination test.  uhttp://ila.ilsl.br/pdfs/v58n2a07.pdf  a328-330 v583 a<p>In this study, we have developed two latex agglutination tests (LATs) with phenolic glycolipid-I (PGL-I) and natural disaccharide-octyl-bovine serum albumin (ND-O-BSA) as antigens in 110 leprosy patients (LL = 30, BL = 30, BT = 30, and TT = 20), 50 tuberculosis cases, and 30 normal controls. These two LATs were compared with corresponding ELISAs (ND-O-BSA ELISA and PGL-I ELISA) and analyzed by the chi-squared test. There were no significant differences between the two LATs (PGL-I LAT and ND-O-BSA LAT) and their corresponding ELISAs. There was an increase in the proportion of positive cases detectable which coincided with the clinical classification of leprosy, i.e., lepromatous cases were more likely to be positive than tuberculoid cases. LATs are more simple and rapid than ELISAs and have high sensitivity (77% in ND-O-BSA LAT, 80.5% in PGL-I LAT) and specificity (99% in both LATs). LATs may become useful tools for the immunodiagnosis of leprosy in the field. The stability and repeatability of LATs are discussed in detail.</p>
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