01797nas a2200337   4500000000100000008004100001260001300042653002600055653002400081653002800105653003800133653001100171653002100182653001200203653002500215653002500240653002100265653003100286100001200317700001700329700001300346700001800359700001500377245021000392856007800602300001100680490000700691050001200698520073500710022001401445       1990                            d  c1990 Feb10aAntibodies, Bacterial10aAntigens, Bacterial10aDiagnosis, Differential10aEnzyme-Linked Immunosorbent Assay10aHumans10aImmunoglobulin G10aleprosy10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aMolecular Weight10aMycobacterium tuberculosis1 aDas P K1 aRambukkana A1 aBaas J G1 aGroothuis D G1 aHalperin M00aEnzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC269615/pdf/jcm00050-0229.pdf  a379-820 v28  aDAS19903 a<p>Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and of 28 tuberculoid leprosy patients reacted in a significant manner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy patients, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguishing two polar types of leprosy and for diagnosing leprosy in general.</p>  a0095-1137