02289nas a2200337   4500000000100000008004100001260001700042653001200059653002600071653002400097653002300121653002300144653001100167653001200178653000900190653002500199653002700224653002400251100002200275700001400297700001400311700001700325700001100342700001700353700001400370245004600384300001100430490000800441520148800449022001401937       1990                            d  c1990 Sep-Oct10aAnimals10aAntibodies, Bacterial10aAntigens, Bacterial10aBacterial Proteins10aBacterial Vaccines10aHumans10aleprosy10aMice10aMycobacterium leprae10aSalmonella typhimurium10aVaccines, Synthetic1 aClark-Curtiss J E1 aThole J E1 aSathish M1 aBosecker B A1 aSela S1 aCarvalho E F1 aEsser R E00aProtein antigens of Mycobacterium leprae.  a859-710 v1413 a<p>Protein antigens of Mycobacterium leprae have been identified by screening the lambda gt11, pYA626 and pHC79::M. leprae genomic libraries with pooled sera from leprosy patients and with antiserum to M. leprae cell wall protein (CWP) aggregate. Immunological screening of the lambda gt11 library with pooled sera from 21 lepromatous (LL) leprosy patients resulted in the identification of 19 antigens that are apparently different from previously identified M. leprae antigens. Five additional antigens were identified by screening the lambda gt11 library with pooled sera from 30 borderline tuberculoid or tuberculoid patients. Four other antigens were identified by screening the lambda gt11 library with anti-CWP. Two groups of recombinant cosmids were identified by screening the pHC79 library with LL patients' sera: one group specified proteins that reacted with monoclonal antibodies (mAb) against the 65-kDa protein and against the 18-kDa protein; the other group specified a 15-kDa protein that did not react with any of the mAb that were tested. One pYA626 clone also specified a 15-kDa protein that reacted with LL patients' sera, but did not react with any mAb. Genes specifying several of these antigens have been subcloned into the Asd+ plasmid vector pYA292 and have been introduced into a delta cya delta crp delta asd Salmonella typhimurium strain to evaluate the ability of individual M. leprae proteins to elicit immune responses against M. leprae infection.</p>  a0923-2508