01987nas a2200301   4500000000100000008004100001260001300042653002700055653002400082653001100106653001100117653004000128653001100168653001900179653001200198653002100210653002500231100001400256700001200270700001300282700001500295245019100310856009000501300001100591490000700602520106200609022001401671       1991                            d  c1991 Jun10aAntibodies, Monoclonal10aAntigens, Bacterial10aAvidin10aBiotin10aElectrophoresis, Polyacrylamide Gel10aHumans10aImmunoblotting10aleprosy10aMolecular Weight10aMycobacterium leprae1 aPatil S A1 aTyagi P1 aKatoch K1 aSengupta U00aAntigens of Mycobacterium leprae in the cerebrospinal fluid of leprosy patients: detection by monoclonal-antibody-based sandwich immunoradiometric assay and avidin/biotin immunoblotting.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1535415/pdf/clinexpimmunol00063-0145.pdf  a515-210 v843 a<p>Mycobacterium leprae antigens could be detected in the cerebrospinal fluid (CSF) of patients with leprosy, using a monoclonal-antibody-based sandwich immunoradiometric assay (SIRMA). Antigens of 12 kD, 35 kD and 30-40 kD were detected using ML06, ML04, and ML34 monoclonal antibodies, respectively. The 30-40-kD polysaccharide antigen, although present in larger amounts in M. leprae than the 12-kD and 35-kD protein antigens, was found in the CSF of comparatively fewer subjects. The antigen capture assay has been found sensitive to the level of nanograms. Avidin-biotin-based immunoblotting using pooled leprosy sera detected a larger number of antigens than using anti-M. leprae antisera raised in rabbits. The immunoblotting of CSF samples revealed about three antigens in the region of 100-160 kD and three more in the region of 45-60 kD as probed by leprosy sera. This study has for the first time revealed the presence of M. leprae antigens in the CSF of leprosy patients and the probable involvement of the central nervous system in leprosy.</p>  a0009-9104