01996nas a2200313   4500000000100000008004100001260001300042653001200055653002300067653001900090653002200109653001200131653002600143653002500169653003600194653001700230100001500247700001600262700001400278700002400292700002100316245009900337856005100436300001000487490000700497050003200504520113200536022001401668       2009                            d  c2009 Sep10aAnimals10aBacterial Proteins10aDNA, Bacterial10aGenetic Variation10aleprosy10aMinisatellite Repeats10aMycobacterium leprae10aPolymorphism, Single Nucleotide10aSigma Factor1 aMatsuoka M1 aGonzalez AV1 aEstrada I1 aCarreño-Martinez C1 aFafutis-Morris M00aVarious genotypes of Mycobacterium leprae from Mexico reveal distinct geographic distribution.  uhttps://leprosyreview.org/article/80/3/32-2326  a322-60 v80  aInfolep Library - available3 a<p><b>OBJECTIVE: </b>To classify Mycobacterium leprae isolates from multiple areas in Mexico based on variable number of tandem repeats of 6 base within the rpoT gene and three single nucleotide polymorphism (SNP), and to analyse their geographic distribution in the context of the origin of leprosy in Mexico.</p><p><b>RESULTS: </b>Analysis for rpoT genotyping of 64 samples collected in the west and southwestern areas revealed that 46 isolates were of the 4 copy type and 18 isolates were of the 3 copy type. All samples from the eastern coastal area (n = 24) and from the Yucatan peninsula (n = 12) were of the 3 copy type. Six isolates from the west and southwestern area were SNP-type 1, 13 isolates were SNP-type 2 and 45 isolates were SNP-type 3. Nineteen of 24 isolates from the eastern coastal area were SNP-type 3 and one was SNP-type 4. Seven isolates from the Yucatan peninsula were SNP-type 3 and one was SNP-type 4.</p><p><b>CONCLUSION: </b>The difference of the proportion of each genotype between the western areas and the eastern areas indicated the expansion of leprosy through different paths in Mexico.</p>  a0305-7518