02168nas a2200373 4500000000100000008004100001260001300042653002300055653003200078653001800110653001600128653002100144653002300165653003300188653001300221653001100234653001300245653001200258653002500270653003000295653004600325100001300371700001700384700001500401700001100416700001400427245014600441856005100587300001100638490000700649050001500656520110900671022001401780 2009 d c2009 Jun10aBacterial Proteins10aBacterial Typing Techniques10aChaperonin 6010aChaperonins10aCluster Analysis10aDNA Fingerprinting10aDNA-Directed RNA Polymerases10agenotype10aHumans10aLepromin10aleprosy10aMycobacterium leprae10apolymerase chain reaction10aPolymorphism, Restriction Fragment Length1 aAlban SM1 aSella SR B R1 aMiranda RN1 aMira M1 aSoccol VT00aPCR-restriction fragment length polymorphism analysis as a tool for Mycobacterium species identification in lepromas for lepromin production. uhttps://leprosyreview.org/article/80/2/12-9142 a129-420 v80 aALBAN 20093 a

OBJECTIVES: The aim of the present work was to standardise a PCR-Restriction Fragment Length Polymorphism analysis (PRA) as a tool to detect the mycobacteriologic composition of lepromas from leprosy patients used in the production of lepromin to improve the quality of the Mitsuda test.

DESIGN: PCR-Restriction Fragment Length Polymorphism analysis using hsp65 and rpoB genes were applied to 11 reference strains of mycobacteria, including M. leprae, and the obtained PRA profiles were compared to mycobacteria in clinical specimens.

RESULTS: Out of the biopsies studied, 522% had DNA fragment amplified for both genes (hsp65 and rpoB) for M. leprae. However, other Mycobacterium species were observed in samples of lepromatous leprosy patients. Here we discussed the importance of mycobacteria identification in the antigen of Mitsuda production to be used in the evaluation of leprosy.

CONCLUSIONS: Our results suggest that the use of the molecular approach for sample selection can contribute to an improvement in the quality of produced lepromin.

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