01723nas a2200373   4500000000100000008004100001260001600042653001800058653001400076653001100090653002100101653002100122653001900143653001800162653001800180653001800198653001200216653002800228653003000256653001900286653000900305100001500314700001400329700001400343700001500357700001200372700001400384700001500398245008600413300001000499490000800509520081800517022001401335       1991                            d  c1991 Oct 1110aBase Sequence10aCytokines10aHumans10aImmunity, Innate10aInterferon-gamma10aInterleukin-1010aInterleukin-210aInterleukin-410aInterleukin-510aleprosy10aMolecular Sequence Data10apolymerase chain reaction10aRNA, Messenger10aSkin1 aYamamura M1 aUyemura K1 aDeans R J1 aWeinberg K1 aRea T H1 aBloom B R1 aModlin R L00aDefining protective responses to pathogens: cytokine profiles in leprosy lesions.  a277-90 v2543 a<p>The immunological mechanisms required to engender resistance have been defined in few infectious diseases of man, and the role of specific cytokines is unclear. Leprosy presents clinically as a spectrum in which resistance correlates with cell-mediated immunity to the pathogen. To assess in situ cytokine patterns, messenger RNA extracted from leprosy skin biopsy specimens was amplified by the polymerase chain reaction with 14 cytokine-specific primers. In lesions of the resistant form of the disease, messenger RNAs coding for interleukin-2 and interferon-gamma were most evident. In contrast, messenger RNAs for interleukin-4, interleukin-5, and interleukin-10 predominated in the multibacillary form. Thus, resistance and susceptibility were correlated with distinct patterns of cytokine production.</p>  a0036-8075