01519nas a2200289   4500000000100000008004100001260001300042653002000055653002200075653002500097653004400122653001600166653001900182100001200201700001300213700001500226700001600241700000900257700001500266700001400281700001200295245010000307300001000407490000700417520079100424022001401215       2009                            d  c2009 Feb10aGene Expression10aGenome, Bacterial10aMycobacterium leprae10aOligonucleotide Array Sequence Analysis10aPseudogenes10aRNA, Bacterial1 aAkama T1 aSuzuki K1 aTanigawa K1 aKawashima A1 aWu H1 aNakamura K1 aHayashi M1 aIshii N00a[Detection of RNA expression on whole genome analysis of Mycobacterium leprae by tiling array].  a49-540 v783 a<p>Completion of Mycobacterium leprae genome sequence revealed that there are many pseudogenes and non-coding regions, but rather small numbers of protein-coding genes. Although it was thought that pseudogenes and non-coding regions were silent and junk, our previous studies indicated that RNA expression was detected from these regions. To elucidate comprehensive RNA expression pattern on M. leprae whole genome, tiling array was designed and total RNA of M. leprae Thai-53 strain was analyzed. As a result, highly expressed regions were detected among not only the gene regions but also pseudogenes and non-coding regions. Since some of the RNA expression levels were modulated by MDT, evaluation of RNA expression pattern might be a good indicator for the treatment of leprosy.</p>  a1342-3681