01749nas a2200325 4500000000100000008004100001260001300042653003100055653002000086653002100106653005300127653001100180653001100191653002000202653001900222653002600241653001600267653002200283653002400305100001300329700001200342700001000354700001300364700001200377245014800389300001000537490000700547520085500554022001401409 2009 d c2009 Jan10aCD4-Positive T-Lymphocytes10aCells, Cultured10aChild, Preschool10aGranulocyte-Macrophage Colony-Stimulating Factor10aHumans10aInfant10aInfant, Newborn10aInterleukin-1010aLymphocyte Activation10aMacrophages10aMembrane Proteins10aMycobacterium bovis1 aMakino M1 aMaeda Y1 aKai M1 aTamura T1 aMukai T00aGM-CSF-mediated T-cell activation by macrophages infected with recombinant BCG that secretes major membrane protein-II of Mycobacterium leprae. a39-460 v553 a

The potential of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) needs to be augmented to efficiently activate CD4(+) T cells through macrophages. Mycobacterium leprae-derived recombinant major membrane protein (MMP)-II induced GM-CSF production from macrophages. A recombinant BCG-SM that secretes MMP-II more efficiently produced GM-CSF and activated interferon (IFN)-gamma-producing CD4(+) T cells than did vector control BCG when infected with macrophages. The T-cell activation by BCG-SM was dependent on the GM-CSF production by macrophages. Interleukin (IL)-10 production by macrophages stimulated with M. leprae was inhibited in a GM-CSF-dependent manner when the precursor monocytes were infected with BCG-SM. BCG inducing GM-CSF production was effective in macrophage-mediated T-cell activation partially through IL-10 inhibition.

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