01546nas a2200289   4500000000100000008004100001260000900042653001900051653001100070653001200081653002500093653001700118653000900135653003000144653003200174100001500206700001600221700001400237700001700251700001400268245005900282856005700341300001000398490001500408520081900423022001401242       2008                            d  c200810aDNA, Bacterial10aHumans10aleprosy10aMycobacterium leprae10aNasal Mucosa10aNose10apolymerase chain reaction10aSensitivity and Specificity1 aPontes ARB1 aAlmeida MGC1 aXavier MB1 aQuaresma JAS1 aYassui EA00a[Detection of Mycobacterium leprae DNA in nasal swab].  uhttp://www.scielo.br/pdf/reben/v61nspe/a13v61esp.pdf  a734-70 v61 Spec No3 a<p>Studies have demonstrated high sensibility of the polimerase chain reaction (PCR) technique in the identification of the Mycobacterium leprae DNA . This study aimed to evalue the PCR sensibility at the detection of the M. leprae DNA in nasal swab of leprosy patients and to compare the results with the bacilloscopy and multibacillary (MBs) and paucibacilares (PBs) forms. Nasal secretion samples of 24 leprosy patients were collected, and were preserved in one and two lise's solution. The PCR results were highly significant (p <0.0000) and they revealed grater sensibility than bacilloscopy, in several clinical forms. Nevertheless, still different studies are necessary, testing new markers and preservatives, with the purpose of lifting up the sensibility of this technique, in nasal secretion samples.</p>  a0034-7167