01746nas a2200301   4500000000100000008004100001260001300042653001200055653002500067653003000092653002700122653001300149653001100162653001200173653002600185653001700211653001300228653003200241100001600273700002000289700001900309700002300328245005100351300001200402490000700414520100900421022001401430       2007                            d  c2007 Jun10aAlleles10aCase-Control Studies10aCation Transport Proteins10aDisease Susceptibility10agenotype10aHumans10aleprosy10aPolymorphism, Genetic10aRisk Factors10aThailand10aTumor Necrosis Factor-alpha1 aVejbaesya S1 aMahaisavariya P1 aLuangtrakool P1 aSermduangprateep C00aTNF alpha and NRAMP1 polymorphisms in leprosy.  a1188-920 v903 a<p><b>OBJECTIVE: </b>To determine the association of TNF alpha and NRAMP1 polymorphisms in leprosy.</p><p><b>MATERIAL AND METHOD: </b>The polymorphisms of TNF alpha at -238, -308, and NRAMP1 at INT4, D543N, and3' UTR were examined in 37 patients with leprosy (24 multibacillary and 13 paucibacillary) and 140 healthy controls. PCR-SSP and PCR-SSO method were used to type TNF and NRAMP1 polymorphisms.</p><p><b>RESULTS: </b>The genotype frequency of TNF-308 G/A was significantly increased in all leprosy patients compared to the controls (p = 0.04, OR = 2.69). When leprosy types were divided, the allele frequency of TNF-308A was significantly increased in multibacillary leprosy compared to the normal controls (p = 0.04, OR = 2.93). There was no significant difference in the distribution of the genotypes and allele frequencies of TNF -238 and NRAMP1 polymorphisms between the patients and controls.</p><p><b>CONCLUSION: </b>TNF-308A was associated with susceptibility to multibacillary leprosy.</p>  a0125-2208