02163nas a2200277   4500000000100000008004100001260001300042653002600055653002400081653003800105653001100143653001200154653002500166653003200191653000900223653002900232100001700261700001400278700001500292700001200307245014800319300001200467490000800479520138400487022001401871       2007                            d  c2007 Jul10aAntibodies, Bacterial10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aHumans10aleprosy10aMycobacterium leprae10aSensitivity and Specificity10aSkin10aSkin Diseases, Bacterial1 aChaturvedi V1 aGirdhar B1 aSengupta U1 aSinha S00aSemi-quantitative detection of Mycobacterium leprae antigens in skin scrapings: suitability as a laboratory aid for field diagnosis of leprosy.  a699-7060 v1013 a<p>We describe here a method, potentially suitable for field applications, for semi-quantitative detection of Mycobacterium leprae antigens in skin scrapings, which are taken normally for smear microscopy. Thirty acid-fast bacilli-negative paucibacillary (PB) leprosy patients comprised the main study group; eight acid-fast bacilli-positive multibacillary (MB) patients and five healthy laboratory workers served as controls. Samples in saline were spotted on nitrocellulose paper and probed with mycobacterium-specific polyclonal or M. leprae-specific mAbs against 12, 35 and 65kDa protein antigens, using a dot-ELISA format. Spot densities were read through a densitometer and also graded visually. The polyclonal antibody produced the best sensitivity, resulting in densitometric detection of mycobacterial antigen in 100% MB, 76% multiple-lesion PB and 62% single-lesion PB patients. None of the healthy volunteers showed antigen positivity. A correlation was noted between the densitometric and visual estimates of the antigen. Determination of antigen in the lesion and an apparently uninvolved area of skin in a subset of PB patients provided clues to the state of the underlying infection. Serological positivity of PB patients for M. leprae-specific antibodies against the 35kDa and phenolic glycolipid-I antigens was too low (<20%) for any diagnostic significance.</p>  a0035-9203