01698nas a2200301   4500000000100000008004100001260001300042653001200055653001200067653003100079653001100110653002300121653001200144653000900156653003200165653001300197653002500210653001400235653004400249653001300293100001300306700001500319245008400334300001000418490000700428520094700435022001401382       2006                            d  c2006 Sep10aAnimals10aDapsone10aDrug Resistance, Microbial10aHumans10aLeprostatic Agents10aleprosy10aMice10aMicrobial Sensitivity Tests10aMutation10aMycobacterium leprae10aOfloxacin10aOligonucleotide Array Sequence Analysis10aRifampin1 aSuzuki Y1 aMatsuoka M00a[DNA microarray based rapid drug susceptibility test for Mycobacterium leprae].  a271-70 v753 a<p>Antibiotic susceptibility test of Mycobacterium leprae still relies on the time consuming methods based on the growth of M. leprae in the mouse footpad. Thus, the establishment of a rapid, simple and reliable method for the detection of drug-resistant M. leprae is one of the most urgent subjects in the treatment of leprosy patients. Recently, many data on the mutation of specific genes correlating with drug resistance have been accumulated. Application of these data permit the establishment of new gene diagnostic methods for drug susceptibility test of leprosy. In this paper, the method using the low density oligonucleotide array that enables the detection of base substitutions involved in resistance against anti-leprosy drugs on a single platform was discussed. The low density oligonucleotide array described in this paper will open the new perspectives in terms of patient management for leprosy with low cost requirement.</p>  a1342-3681