02016nas a2200385   4500000000100000008004100001260001300042653002400055653001800079653003100097653002000128653001600148653004000164653002000204653003100224653001100255653002100266653001200287653000900299653002800308653002500336653004300361653005200404100001200456700001200468700001500480700001000495700001400505245010200519856007700621300001100698490000800709520089900717022001401616       2007                            d  c2007 Mar10aAntigens, Bacterial10aBase Sequence10aCD4-Positive T-Lymphocytes10aCells, Cultured10aClone Cells10aComplementarity Determining Regions10aHLA-DR Antigens10aHistocompatibility Testing10aHumans10aInterferon-gamma10aleprosy10aMale10aMolecular Sequence Data10aMycobacterium leprae10aReceptors, Antigen, T-Cell, alpha-beta10aReverse Transcriptase Polymerase Chain Reaction1 aSabet S1 aOchoa M1 aSieling PA1 aRea T1 aModlin RL00aFunctional characterization of a T-cell receptor BV6+ T-cell clone derived from a leprosy lesion.  uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265884/pdf/imm0120-0354.pdf  a354-610 v1203 a<p>Human infection with Mycobacterium leprae, an intracellular bacterium, presents as a clinical and immunological spectrum; thus leprosy provides an opportunity to investigate mechanisms of T-cell responsiveness to a microbial pathogen. Analysis of the T-cell receptor (TCR) repertoire in leprosy lesions revealed that TCR BV6(+) T cells containing a conserved CDR3 motif are over-represented in lesions from patients with the localized form of the disease. Here, we derived a T-cell clone from a leprosy lesion that expressed TCR BV6 and the conserved CDR3 sequence L-S-G. This T-cell clone produced a T helper type 1 cytokine pattern, directly lysed M. leprae-pulsed antigen-presenting cells by the granule exocytosis pathway, and expressed the antimicrobial protein granulysin. BV6(+) T cells may therefore functionally contribute to the cell-mediated immune response against M. leprae.</p>  a0019-2805