02088nas a2200409   4500000000100000008004100001260001300042653002700055653001700082653004600099653004400145653001600189653002000205653001900225653001100244653002200255653002400277653001300301653001200314653003300326653002400359653002600383653002500409653001600434653003100450653001800481100001300499700001700512700001400529700001300543700001300556245011400569300001000683490000700693520096400700022001401664       1990                            d  c1990 Feb10aAntibodies, Monoclonal10aAntigens, CD10aAntigens, Differentiation, Myelomonocytic10aAntigens, Differentiation, T-Lymphocyte10aCD3 Complex10aDNA Replication10aFlow Cytometry10aHumans10aImmunosuppression10aIn Vitro Techniques10aKinetics10aleprosy10aLipopolysaccharide Receptors10aLipopolysaccharides10aLymphocyte Activation10aMycobacterium leprae10aPolymyxin B10aReceptors, Antigen, T-Cell10aT-Lymphocytes1 aMolloy A1 aGaudernack G1 aLevis W R1 aCohn Z A1 aKaplan G00aSuppression of T-cell proliferation by Mycobacterium leprae and its products: the role of lipopolysaccharide.  a973-70 v873 a<p>Addition of soluble molecules obtained from sonicated Mycobacterium leprae markedly suppressed the proliferative response to the mitogen anti-CD3 of peripheral blood mononuclear cells and isolated T cells. Suppression was nonspecific and occurred with cells from lepromatous and tuberculoid leprosy patients as well as control donors. The purified lipoarabinomannans from M. leprae and Mycobacterium tuberculosis had a similar spectrum of inhibition whereas their deacylated derivatives were without effect. All mycobacterial preparations of either a crude or purified state, which suppressed cellular responses, contained appreciable quantities of bacterial lipopolysaccharide by the Limulus amebocyte assay. Contamination with lipopolysaccharide could account for the extent and nonselectivity of the T-cell suppression. Suppression was also monocyte-dependent and in part due to the release of arachidonate metabolites of the cyclooxygenase pathway.</p>  a0027-8424