01263nas a2200265   4500000000100000008004100001260001300042653002100055653001100076653003100087653002100118653001100139653002300150653001200173653002500185653003000210653001300240100001500253700001300268700001600281245010300297300001000400490000600410520058100416       2006                            d  c2006 Jun10aBiological Assay10aBiopsy10aDrug Resistance, Bacterial10aGenes, Bacterial10aHumans10aLeprostatic Agents10aleprosy10aMycobacterium leprae10apolymerase chain reaction10aRifampin1 aSapkota BR1 aRanjit C1 aMacdonald M00aReverse line probe assay for the rapid detection of rifampicin resistance in Mycobacterium leprae.  a122-70 v83 a<p>Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.</p>