01669nas a2200337   4500000000100000008004100001260001300042653001500055653002400070653001900094653001600113653001100129653001200140653001100152653000900163653002500172653001800197653001900215100001600234700001500250700002100265700002000286700001400306245018500320856005900505300001000564490000700574050003200581520070400613022001401317       1992                            d  c1992 Jun10aAdolescent10aAntigens, Bacterial10aCarbon Dioxide10aGlycolipids10aHumans10aleprosy10aLipids10aMale10aMycobacterium leprae10aPalmitic Acid10aPalmitic Acids1 aShannon E J1 aHarris E B1 aHaile-Mariam H S1 aGuebre-Xavier M1 aFrommel D00aCompetency of human-derived Mycobacterium leprae to use palmitic acid in the synthesis of phenolic glycolipid-I and phthiocerol dimycocerosate and to release CO2 in axenic culture.  uhttp://leprev.ilsl.br/pdfs/1992/v63n2/pdf/v63n2a02.pdf  a101-70 v63  aInfolep Library - available3 a<p>Insufficient numbers of viable Mycobacterium leprae have hampered metabolic studies using human-derived M. leprae. In this study, sufficient numbers of M. leprae were obtained from an untreated lepromatous patient to titrate the effects of pH on the metabolism of 14C-palmitic acid by M. leprae. Catabolic metabolism (oxidation of 14C-palmitic acid and release of 14CO2) was maximal when M. leprae were incubated at 33 degrees C and suspended in Middlebrook 7H9, ADC supplemented medium that had been buffered to maintain a pH of 4.8. Anabolic metabolism (synthesis of 14C-phenolic glycolipid-I and its precursor, 14C-phthiocerol dimycocerosate) was maximal when the pH was maintained at 6.8.</p>  a0305-7518