01597nas a2200277 4500000000100000008004100001260001300042653002400055653002400079653003800103653003200141653001600173653001100189653001200200653002500212653003200237100001200269700001400281245011100295856005900406300001100465490000700476050003200483520079000515022001401305 1992 d c1992 Jun10aAgglutination Tests10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aEvaluation Studies as Topic10aGlycolipids10aHumans10aleprosy10aMycobacterium leprae10aSensitivity and Specificity1 aSekar B1 aAnandan D00aEvaluation of Mycobacterium leprae particle agglutination test, using eluates of filter paper blood spots. uhttp://leprev.ilsl.br/pdfs/1992/v63n2/pdf/v63n2a04.pdf a117-240 v63 aInfolep Library - available3 a
A comparison of the ELISA test with the newly-developed MLPA test was carried out, using eluates of blood spots from filter paper for the detection of the anti-PGL-I antibody. A very good positive correlation was observed between these two tests. The concordance rate was found to be 92.6%, ranging from 71.4% to 100%. This nonconcordance was not found when freshly-collected samples were used. The MLPA test is simple and reliable. The use of eluates from blood spots collected on filter paper further simplifies the test in the collection and transportation of blood samples. This accurate and rapid method makes the MLPA test logistically feasible for large-scale screening. With our modification of MLPA with eluates more samples can be screened with the kit than with sera.
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