01410nas a2200289   4500000000100000008004100001260000900042653001200051653004000063653003800103653001100141653001900152653002000171653001200191653001000203653002500213653001200238653000900250100001800259700001300277700001700290245007100307300001000378490000700388520071100395022001401106       1992                            d  c199210aAnimals10aElectrophoresis, Polyacrylamide Gel10aEnzyme-Linked Immunosorbent Assay10aHumans10aImmunoblotting10aImmunodiffusion10aleprosy10aLiver10aPhosphatidylcholines10aRabbits10aRats1 aDeshpande R G1 aKhan M B1 aNavalkar R G00aImmunoreactivity of a mammalian liver component with leprosy sera.  a345-90 v973 a<p>Sera from 77 leprosy patients in various stages of infection--tuberculoid (TT), lepromatous (LL), borderline tuberculoid and borderline lepromatous--15 contacts and 21 normal healthy individuals, were assayed in an indirect enzyme-linked immunosorbent assay and dot enzyme immunoassay using ethanol-soluble and thermostable extract of liver as the antigen. The highest incidences of reaction were found in untreated LL patients (100%) and in TT patients (91%), while the sera from borderline patients showed a comparatively lower incidence (43%). Some of the sera from contacts of leprosy patients (6/15) also showed high reactivity. Assays using lecithin as an antigen did not exhibit any reaction.</p>  a1018-2438