02920nas a2200397   4500000000100000008004100001260001300042653002400055653002000079653004200099653004000141653001100181653002300192653002500215653001200240653002400252653002500276653002500301653002600326653002500352653001400377653001800391100001200409700001300421700001700434700001300451700001200464700001700476245012100493856004100614300001000655490000700665050001400672520182200686022001402508       1992                            d  c1992 Mar10aAntigens, Bacterial10aCells, Cultured10aElectrophoresis, Gel, Two-Dimensional10aElectrophoresis, Polyacrylamide Gel10aHumans10aImmunity, Cellular10aIsoelectric Focusing10aleprosy10aLeprosy, Borderline10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aLymphocyte Activation10aMycobacterium leprae10aPrognosis10aT-Lymphocytes1 aGulle H1 aSchoel B1 aChiplunkar S1 aGangal S1 aDeo M G1 aKaufmann S H00aT-cell responses of leprosy patients and healthy contacts toward separated protein antigens of Mycobacterium leprae.  uhttp://ila.ilsl.br/pdfs/v60n1a07.pdf  a44-530 v60  aGULLE19923 a<p>Sonicated extracts of Mycobacterium leprae were separated by two-dimensional gel electrophoresis and electroeluted into 400 distinct soluble fractions. These fractions were probed with T lymphocytes from leprosy patients of different disease types, healthy contacts, and unexposed healthy individuals. Proliferative responses were visualized using three-dimensional stimulation profiles. T cells from many patients and contacts responded to a multitude of antigen fractions of different molecular masses and isoelectric points. T cells from unexposed individuals gave significant responses to lysates or whole organisms of M. leprae, but no or only marginal responses to separated antigen fractions. T cells of polar tuberculoid (TT) and the majority of polar lepromatous (LL) leprosy patients responded only to separated antigen fractions but not to lysates or whole organisms of M. leprae. The remaining LL patients were totally unresponsive and even failed to respond to separated M. leprae fractions. Thus, in some leprosy patients unresponsiveness to M. leprae seems to be caused by distinct components and can be broken by using separated antigen fractions; whereas in others, anergy remains. T cells of borderline tuberculoid (BT) patients, who were under chemotherapy, responded to separated antigen fractions as well as to lysates of M. leprae organisms. In contrast, BT patients who were untreated failed to react with any of the M. leprae preparations. Similarly, T cells of the majority of LL patients responding to separated fractions were under chemotherapy; whereas T cells from untreated LL patients gave no or only marginal responses to any of the M. leprae antigen preparations. These findings suggest some linkage between the degree of T-cell responsiveness and antileprosy drug treatment.</p>
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