01658nas a2200301   4500000000100000008004100001260000900042653001200051653001800063653001500081653001100096653002000107653001200127653002000139653000900159653001500168653003700183653001100220653001800231100001500249700001300264700001400277245011300291300001100404490000800415520091900423022001401342       1992                            d  c199210aAnimals10aB-Lymphocytes10aCell Count10aFemale10aImmunoglobulins10aleprosy10aLeukocyte Count10aMice10aMice, SCID10aSevere Combined Immunodeficiency10aSpleen10aT-Lymphocytes1 aXabier M G1 aHowe R C1 aFrommel D00a[Infection and elimination of Mycobacterium leprae in SCID C.B.-17 mice (severe combined immunodeficiency)].  a99-1030 v3143 a<p>Previous studies documented that T-cell deficient nude mice failed to control M. leprae infection. In the present investigation we monitored the growth of M. leprae for up to 15 months in the SCID C.B.-17 mouse, a host deficient in both T and B lymphocytes. At 8 months post-infection 10(8) organisms/foot-pad were recovered from SCID mice vs 5 x 10(6) in normal BALB/c mice. Thereafter the number of bacilli decreased rapidly in mice infected with high-dose inoculum (10(7)); however, at all doses SCID mice eventually cleared M. leprae. During infection both T and B cells as well as serum Ig remained as low as in uninfected mice; however, in the spleen MAC-1+ cells which include macrophages and NK cells were substantially increased. These results suggest that MAC-1+ cells are involved in the anti-mycobacteria-1 defence mechanisms adopted by SCID mice to compensate their deficiency in T and B cells.</p>  a0764-4469