01612nas a2200385   4500000000100000008004100001260001300042653001000055653003200065653001900097653002200116653002900138653001100167653001200178653000900190653001600199653003200215653004900247653002500296653004200321653003000363653002400393653002900417653002700446100001400473700001200487700001800499700001500517245012400532856007300656300001100729490000700740520046500747022001401212       2005                            d  c2005 Apr10aAdult10aBacterial Typing Techniques10aDNA, Bacterial10aDiagnostic Errors10aFalse Positive Reactions10aHumans10aleprosy10aMale10aMiddle Aged10aMycobacterium avium Complex10aMycobacterium avium-intracellulare Infection10aMycobacterium leprae10aNucleic Acid Amplification Techniques10apolymerase chain reaction10aRNA, Ribosomal, 16S10aReagent Kits, Diagnostic10aSequence Analysis, DNA1 aLefmann M1 aMoter A1 aSchweickert B1 aGöbel U B00aMisidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare test.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081340/pdf/1425-04.pdf  a1928-90 v433 a<p>Commercially available nucleic acid probe- and amplification-based systems for detection and differentiation of mycobacteria are widely used in clinical microbiology laboratories. Here we report two cases of human leprosy in which the COBAS AMPLICOR Mycobacterium intracellulare test led to false- positive results. Correct identification of Mycobacterium leprae was possible only by amplification and comparative sequence analysis of the 16S rRNA gene.</p>  a0095-1137