01493nas a2200289   4500000000100000008004100001260001300042653001100055653001200066653002800078653002900106653002400135653002500159653001700184653002500201653002200226653003200248100001400280700001200294700001200306700001600318245011900334300000900453490000600462520072100468022001401189       1992                            d  c1992 Jan10aHumans10aleprosy10aLeukocytes, Mononuclear10aLuminescent Measurements10aMycobacterium bovis10aMycobacterium leprae10aPhagocytosis10aRecombinant Proteins10aRespiratory Burst10aTumor Necrosis Factor-alpha1 aLaunois P1 aNiang M1 aDieye A1 aSarthou J L00aTNF-alpha failed to reverse the M. leprae-induced defective chemiluminescence response of human mononuclear cells.  a91-60 v43 a<p>The effect of phagocyte activation by TNF-alpha on the ability to trigger a chemiluminescence (CL) response, associated with the release of oxidizing species was evaluated in healthy human mononuclear cells in the presence of Mycobacterium leprae. Recombinant TNF-alpha (r-TNF-alpha) increased the CL response of unstimulated M. bovis BCG- and PMA-stimulated cells but did not reverse the M. leprae defective activation of the human phagocyte oxidative burst. M. leprae was less well phagocytosed than M. bovis BCG but phagocytosis of mycobacteria was not altered by addition of r-TNF-alpha. The failure of activation of oxygen-free radical production might have some relevance to the pathogenesis of leprosy.</p>  a0920-8534