01967nas a2200421   4500000000100000008004100001260001700042653005200059653001200111653002300123653001900146653003100165653002100196653001300217653001100230653001000241653001200251653002500263653002400288653002700312653001700339653000900356653002800365100001400393700001000407700001300417700001400430700001400444700001400458700001500472245010900487856007800596300001100674490000700685050003200692520080700724022001401531       2009                            d  c2009 Jul-Sep10aAmplified Fragment Length Polymorphism Analysis10aAnimals10aBacterial Proteins10aDNA, Bacterial10aEmigration and Immigration10aGenes, Bacterial10agenotype10aHumans10aIndia10aleprosy10aMycobacterium leprae10aPopulation Dynamics10aSequence Analysis, DNA10aSigma Factor10aSkin10aTandem Repeat Sequences1 aLavania M1 aLal R1 aJoseph G1 aDarlong J1 aAbraham S1 aNanda N K1 aJadhav R S00aGenotypic analysis of Mycobacterium leprae strains from different regions of India on the basis of rpoT.  uhttp://www.ijl.org.in/July-Sept%202009_PDF%20Files/2_Abst%20(119-124).pdf  a119-240 v81  aInfolep Library - available3 a<p>Mycobacterium leprae strains from Indian leprosy patients were analyzed using the six base tandem repeat, GACATC, in rpoT gene as genetic marker. DNA was extracted from slit-skin smears and nasal swabs of new untreated as well as treated leprosy patients living in different regions of India. PCR amplification of rpoT gene and sequencing of amplicons showed the presence of two genotype of M. leprae in this study, 73.4% having three copies (ancient Indian type) and 26.6% contain 4 copies (considered to be Japanese and Korean). These genotypes along with other short tandem repeats may help in studying the historical spread of disease and the strains of M. leprae disseminated by various human races that migrated to India from other places of Asia and European countries during our history.</p>  a0254-9395