01597nas a2200289   4500000000100000008004100001260001300042653001200055653002600067653001500093653001100108653002900119653001100148653002500159653001200184653002400196653002500220100001200245700001700257700002500274245016800299856004100467300001100508490000700519520076700526022001401293       2004                            d  c2004 Sep10aAnimals10aAntibodies, Bacterial10aArmadillos10aBrazil10aFalse Positive Reactions10aHumans10aImmunohistochemistry10aleprosy10aMycobacterium bovis10aMycobacterium leprae1 aDeps PD1 aMichalany NS1 aTomimori-Yamashita J00aFalse positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos.  uhttp://ila.ilsl.br/pdfs/v72n3a11.pdf  a327-300 v723 a<p>The authors studied 66 wild nine-banded armadillos from Brazil. The ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. The AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many normal structures from the ears of the nine-banded armadillos, such as condrocytes, condroblasts, fibroblasts and endothelial cells, and Gram positive bacteria cocci, showed false positive reaction by the BCG marker. The authors discuss the use of the immunohistochemical studies with the polyclonal BCG antibody to identify M. leprae antigens in wild armadillos.</p>  a0148-916X