02067nas a2200337   4500000000100000008004100001260001300042653002300055653004000078653001100118653001100129653002300140653001200163653002500175653002500200653002600225653002100251653002500272653001500297653001600312100001300328700001600341700001500357700001300372245010900385856004100494300001100535490000700546520116200553022001401715       1992                            d  c1992 Jun10aBacterial Proteins10aElectrophoresis, Polyacrylamide Gel10aFamily10aHumans10aImmunity, Cellular10aleprosy10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aLymphocyte Activation10aMolecular Weight10aMycobacterium leprae10aSolubility10aVaccination1 aRada E M1 aSantaella C1 aAranzazu N1 aConvit J00aPreliminary study of cellular immunity to Mycobacterium leprae protein in contacts and leprosy patients.  uhttp://ila.ilsl.br/pdfs/v60n2a04.pdf  a189-940 v603 a<p>Because of the good results obtained in the mononuclear cell (T lymphocyte) proliferative response in tuberculoid leprosy patients and family contacts and healthy Mitsuda-positive volunteers using Mycobacterium leprae soluble extract, we prepared different protein fractions from the soluble extract. We used the T-cell Western blot technique with separation by electrophoresis in SDS-polyacrylamide gels and transfer onto nitrocellulose membranes. Each unstained blot was converted into 18 fractions of antigen-bearing particles and tested with peripheral blood mononuclear cells from 21 individuals including Mitsuda-positive contacts, vaccinated lepromatous leprosy (LL) patients, borderline tuberculoid (BT) patients, and unvaccinated lepromatous patients. The stimulation index (SI) of the contacts was higher to the different fractions in comparison with the leprosy patients. They showed four peaks of stimulation to fractions 66-55, 45-29, 22-18, and 14 kDa. The second highest responders were BT patients, followed by vaccinated LL patients. The unvaccinated patients did not respond significantly to any of the fractions (SI less than 1).</p>
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