02373nas a2200445   4500000000100000008004100001260001300042653001000055653000900065653001200074653001800086653001100104653001100115653001900126653001900145653001200164653002800176653000900204653002700213653000900240653001500249653001600264653002800280653001900308653002800327653002500355653002400380653003200404100001200436700001200448700001100460700001000471700001100481245014600492300001100638490000800649050001400657520124200671022001401913       2004                            d  c2004 Aug10aAdult10aAged10aAnimals10aBase Sequence10aFemale10aHumans10aInterleukin-1010aInterleukin-1210aleprosy10aLeukocytes, Mononuclear10aMale10aMembrane Glycoproteins10aMice10aMice, Nude10aMiddle Aged10aMolecular Sequence Data10aPoint Mutation10aReceptors, Cell Surface10aToll-Like Receptor 210aToll-Like Receptors10aTumor Necrosis Factor-alpha1 aKang TJ1 aYeum CE1 aKim BC1 aYou E1 aChae G00aDifferential production of interleukin-10 and interleukin-12 in mononuclear cells from leprosy patients with a Toll-like receptor 2 mutation.  a674-800 v112  aKANG 20043 a<p>Toll-like receptor 2 (TLR2) is a key mediator of the immune response to mycobacterial infections, and mutations in TLR2 have been shown to confer susceptibility to infection with mycobacteria. This study investigated the profiles of cytokines, such as interferon (IFN)-gamma, interleukin (IL)-10, IL-12 and tumour necrosis factor (TNF)-alpha in response to Mycobacterium leprae in peripheral blood mononuclear cells (PBMC) with the TLR2 mutation Arg677Trp, a recently reported polymorphism that is associated with lepromatous leprosy. In leprosy patients with the TLR2 mutation, production of IL-2, IL-12, IFN-gamma, and TNF-alpha by M. leprae-stimulated PBMC were significantly decreased compared with that in groups with wild-type TLR2. However, the cells from patients with the TLR2 mutation showed significantly increased production of IL-10. There was no significant difference in IL-4 production between the mutant and wild-type during stimulation. Thus, these results suggest that the TLR2 signal pathway plays a critical role in the alteration of cytokine profiles in PBMC from leprosy patients and the TLR2 mutation Arg677Trp provides a mechanism for the poor cellular immune response associated with lepromatous leprosy.</p>  a0019-2805