02198nas a2200409   4500000000100000008004100001260001300042653002600055653001100081653001600092653001200108653001500120653001900135653003000154653003000184653002400214653001100238653001200249653002500261653003100286653003000317653001300347653003200360653000900392100001700401700001500418700001300433700001200446700001500458700001100473700001200484245013200496300001100628490000600639520112900645022001401774       1992                            d  c1992 Oct10aAnti-Bacterial Agents10aBiopsy10aClofazimine10aDapsone10aDNA Probes10aDNA, Bacterial10aDrug Therapy, Combination10aElectrophoresis, Agar Gel10aFeasibility Studies10aHumans10aleprosy10aMycobacterium leprae10aNucleic Acid Hybridization10apolymerase chain reaction10aRifampin10aSensitivity and Specificity10aSkin1 aWilliams D L1 aGillis T P1 aFiallo P1 aJob C K1 aGelber R H1 aHill C1 aIzumi S00aDetection of Mycobacterium leprae and the potential for monitoring antileprosy drug therapy directly from skin biopsies by PCR.  a401-100 v63 a<p>An improved protocol for PCR analysis of Mycobacterium leprae-infected tissues, based on enzymatic lysis, has been developed and used to demonstrate the feasibility of using PCR for detecting M. leprae in routine skin biopsies taken from leprosy patients throughout the clinical spectrum. Of 92 multibacillary patients tested, 99% were PCR-positive using gel electrophoresis or DNA hybridization to detect the amplified product. Similar analysis of paucibacillary patients, in which only one of 27 biopsies had demonstrable AFB microscopically, gave a positivity rate of 74%. No PCR signals were demonstrated from skin biopsies from seven patients with non-leprosy dermatoses and one AIDS patient with a disseminated atypical mycobacteriosis. Evaluation of leprosy patients with antileprosy drug therapy prior to biopsy demonstrated that PCR signals were either greatly diminished or absent after 2 months of continuous antibiotic therapy. PCR was also able to detect the presence of M. leprae in tissues of patients receiving antibacterial therapy when patients were suspected of harbouring drug-resistant M. leprae.</p>  a0890-8508