02495nas a2200349   4500000000100000008004100001260001300042653002400055653002300079653001800102653001400120653003700134653001100171653002200182653002500204653002800229653002500257653001400282653003100296653000900327653001800336100001400354700001100368700001400379700001200393700001500405245007400420300001100494490000700505520161900512022001402131       1992                            d  c1992 Dec10aAmino Acid Sequence10aBacterial Vaccines10aBase Sequence10aCell Line10aGene Rearrangement, T-Lymphocyte10aHumans10aIntradermal Tests10aLeprosy, Tuberculoid10aMolecular Sequence Data10aMycobacterium leprae10aPhenotype10aReceptors, Antigen, T-Cell10aSkin10aT-Lymphocytes1 aUyemura K1 aHo C T1 aOhmen J D1 aRea T H1 aModlin R L00aSelective expansion of V delta 1 + T cells from leprosy skin lesions.  a848-520 v993 a<p>T cells bearing gamma delta T-cell receptors (TCRs) are prominent residents of murine epidermis and appear to be important participants in the immune response to infection in human skin. The Mitsuda reaction in leprosy, induced by intradermal challenge with Mycobacterium leprae, provides an opportunity to study the cellular events that mediate a form of delayed-type hypersensitivity (DTH) in skin. T cells bearing gamma delta TCRs comprise a significant proportion of the T-cell population in these DTH reactions. Presently we have generated T-cell lines from Mitsuda reactions in vitro and compared their TCR repertoire to that found in situ. gamma delta T cells comprised 20-40% of lines derived from these skin lesions, but < 10% of lines derived from the peripheral blood of the same individuals. Flow-cytometric analysis of variable (V) chain usage in T-cell lines derived from skin lesions indicated that V delta 1 was predominant. Evaluation of the TCR repertoire using PCR indicated that V delta 1-J delta 1 and V gamma 2-J gamma P gene rearrangements were prevalent. In comparison, V delta 2-J delta 1 gene rearrangements predominated in situ. Furthermore, nucleotide sequence analysis of the V-J junction of one T-cell line revealed limited genetic diversity of the gamma delta TCR. These findings suggest that the V delta 1 subpopulation of gamma delta T cells in Mitsuda skin reactions selectively outgrows from leprosy skin lesions in vitro. Such V delta 1 + T-cell lines should be useful for determining the relevant antigens and restriction elements in this response to a pathogen in skin.</p>  a0022-202X