02823nas a2200409   4500000000100000008004100001260001300042653003100055653003100086653001900117653001900136653001500155653001100170653002500181653002600206653001800232653001200250653001600262653001900278653002000297653002000317653002500337653003000362653000900392653003000401100001700431700001600448700002300464700001500487700001600502245009500518856007800613300001100691490000800702520168900710022001402399       2003                            d  c2003 Dec10aCD4-Positive T-Lymphocytes10aCD8-Positive T-Lymphocytes10aChemokine CCL210aChemokine CCL510aChemokines10aHumans10aImmunohistochemistry10aIn Situ Hybridization10aInterleukin-810aleprosy10aMacrophages10aRNA, Messenger10aReceptors, CCR210aReceptors, CCR510aReceptors, Chemokine10aReceptors, Interleukin-8B10aSkin10aStatistics, Nonparametric1 aKirkaldy A A1 aMusonda A C1 aKhanolkhar-Young S1 aSuneetha S1 aLockwood DN00aExpression of CC and CXC chemokines and chemokine receptors in human leprosy skin lesions.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1808882/pdf/cei0134-0447.pdf  a447-530 v1343 a<p>We have investigated the expression of chemokines and their receptors in leprosy skin lesions using immunohistochemistry. Skin biopsies from 25 leprosy patients across the leprosy spectrum, 11 patients undergoing type I reversal reactions and four normal donors were immunostained by ABC peroxidase method using antibodies against CC and CXC chemokines and their receptors. Using an in situ hybridization technique we have also studied the expression of monocyte chemoattractant protein 1 (MCP-1), RANTES and interleukin (IL)-8 chemokines mRNA in leprosy skin lesions. Chemokines and receptor expression was detected in all leprosy skin biopsies. Expression of CC chemokines MCP-1 (P < 0.01) and RANTES (P < 0.01) were elevated significantly in borderline tuberculoid leprosy in reversal reaction compared to non-reactional borderline tuberculoid leprosy, but there was no difference in the expression of IL-8 chemokine. Surprisingly, there was no significant difference in the expression of CC (CCR2 and CCR5) and CXC (CXCR2) chemokine receptors across the leprosy spectrum. Similarly, there was no significant difference in the expression of mRNA for MCP-1, regulated upon activation normal T cell expressed and secreted (RANTES) and IL-8 chemokines. Here, the presence of a neutrophil chemoattractant IL-8 in leprosy lesions, which do not contain neutrophils, suggests strongly a role of IL-8 as a monocyte and lymphocyte recruiter in leprosy lesions. These results suggest that the chemokines and their receptors, which are known to chemoattract T lymphocytes and macrophages, are involved in assembling the cellular infiltrate found in lesions across the leprosy spectrum.</p>  a0009-9104