01991nas a2200397   4500000000100000008004100001260001300042653002400055653001200079653002400091653002300115653002000138653001100158653002100169653001200190653002600202653002800228653002500256653003100281653002200312653001800334653001700352100001200369700002000381700002000401700001300421700001400434700001400448700001200462700002000474245011900494300001000613490000700623520094900630022001401579       2004                            d  c2004 Jan10aAmino Acid Sequence10aAnimals10aAntigens, Bacterial10aBacterial Proteins10aCross Reactions10aHumans10aInterferon-gamma10aleprosy10aLymphocyte Activation10aMolecular Sequence Data10aMycobacterium leprae10aMycobacterium tuberculosis10aSequence Homology10aT-Lymphocytes10aTuberculosis1 aGeluk A1 aMeijgaarden K E1 aFranken K L M C1 aWieles B1 aArend S M1 aFaber W R1 aNaafs B1 aOttenhoff T H M00aImmunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis.  a66-700 v593 a<p>Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guérin (BCG) strains but present in M. leprae, M. tuberculosis, M. bovis, M. kansasii, M. africanum and M. marinum. In this study, the homologue of CFP-10 in M. leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M. leprae is a potent antigen that crossreacts with CFP-10 of M. tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases.</p>  a0300-9475