01629nas a2200301   4500000000100000008004100001260001300042653004100055653001600096653002000112653001100132653001100143653002300154653001200177653000900189653003100198653003200229100001800261700001600279700001600295700001700311700001500328245009600343300001100439490000700450520085600457022001401313       2003                            d  c2003 Sep10aChromatography, High Pressure Liquid10aClofazimine10aDrug Monitoring10aFemale10aHumans10aLeprostatic Agents10aleprosy10aMale10aReproducibility of Results10aSensitivity and Specificity1 aQueiroz R H C1 aPereira R C1 aGotardo M A1 aCordeiro D S1 aMelchior E00aDetermination of clofazimine in leprosy patients by high-performance liquid chromatography.  a377-800 v273 a<p>An original, simple, specific, and rapid high-performance liquid chromatography assay for the determination of clofazimine in human plasma is presented. The procedure consists of extracting the drug and the internal standard (medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (1:1, v/v) at pH 3.0, after precipitating the proteins with methanol. The drugs were then quantitated on a reversed-phase C8 using a mobile phase consisting of a mixture of methanol/0.25 N sodium acetate buffer at pH 3.0 (74:26, v/v). The flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The precision, linearity, and limit of quantitation of the method were within acceptable limits. The method was considered adequate and could be applied in studies involving blood level monitoring and pharmacokinetics in leprosy patients.</p>  a0146-4760