01401nas a2200301   4500000000100000008004100001260001300042653002700055653003700082653001100119653002400130653002500154653002500179653001300204653002000217100001500237700001100252700001100263700001300274700001600287700001200303700001600315245010200331300001000433490000700443520063500450022001401085       1992                            d  c1992 May10aAdenosine Triphosphate10aCentrifugation, Density Gradient10aHumans10aLeprosy, Borderline10aLeprosy, lepromatous10aMycobacterium leprae10aPovidone10aSilicon Dioxide1 aSharma V K1 aKaur S1 aKaur I1 aThakur M1 aVaishnavi C1 aKohli M1 aGanguly N K00aAdenosine triphosphate content of Mycobacterium leprae by percoll buoyant density centrifugation.  a451-30 v303 a<p>Thirty nine untreated patients of bacilliferous leprosy with a mean bacteriological index of 4.8 and morphological index of 1.3% formed the study group. Adenosine triphosphate assay was carried out by (i) enzyme treatment method in 18 patients and (ii) percoll buoyant density gradient method in 21 patients. ATP content obtained by percoll buoyant density gradient method was significantly higher than that obtained by enzyme treatment method. Percoll buoyant density centrifugation for purification and isolation of bacilli from human leproma is simplier, quicker and can serve as an alternate method of enzyme treatment.</p>  a0019-5189