02059nas a2200433   4500000000100000008004100001260001300042653001500055653001000070653000900080653001200089653001000101653002100111653001900132653001300151653001100164653001100175653001100186653001500197653001500212653002900227653000900256653001600265653003000281653004600311653002400357100001300381700001300394700001100407700001300418700001200431700001300443700001300456245012700469300001100596490000800607520099600615022001401611       2007                            d  c2007 Apr10aAdolescent10aAdult10aAged10aAnimals10aChild10aChild, Preschool10aDNA, Protozoan10aEthiopia10aFemale10aHumans10aInfant10aIsoenzymes10aLeishmania10aLeishmaniasis, Cutaneous10aMale10aMiddle Aged10apolymerase chain reaction10aPolymorphism, Restriction Fragment Length10aSpecies Specificity1 aGadisa E1 aGenetu A1 aKuru T1 aJirata D1 aDagne K1 aAseffa A1 aGedamu L00aLeishmania (Kinetoplastida): species typing with isoenzyme and PCR-RFLP from cutaneous leishmaniasis patients in Ethiopia.  a339-430 v1153 a<p>Cutaneous leishmaniasis (CL) is an increasing public health problem in Ethiopia. There is a concern that it is spreading with increased incidence. In this study, we used isoenzyme electrophoresis and internal transcribed spacer one (ITS1) PCR-RFLP techniques to identify Leishmania species from CL patients in Ethiopia. We obtained isolates from 55 localized cutaneous leishmaniasis (LCL), 3 diffused cutaneous leishmaniasis (DCL) and 36 biopsy samples from 34 LCL and 2 DCL cases from All Africa Leprosy and Tuberculosis Rehabilitation and Training Center (ALERT) and clinically diagnosed CL cases from Ochollo village. Both isoenzyme and ITS1 PCR-RFLP techniques showed that Leishmania aethiopica (L. aethiopica) was the aetiologic agent in all cases. Our study also showed that ITS1 PCR-RFLP could identify Leishmania species from biopsy samples and suggests the method could be used for epidemiological surveillance of leishmaniasis in Ethiopia and for species-specific diagnosis.</p>  a0014-4894