01243nas a2200241 4500000000100000008004100001260001300042653001200055653001500067653001800082653001500100653001200115653002500127653002100152100001700173700002000190245012800210300001000338490000700348050001700355520061500372022001400987 1992 d c1992 Nov10aAnimals10aArmadillos10aCulture Media10aGamma Rays10aleprosy10aMycobacterium leprae10aUltraviolet Rays1 aDastidar S G1 aChakrabarty A N00aViability of acid-fast bacilli from gamma-& UV-irradiated lepromatous armadillo tissues infected with Mycobacterium leprae. a263-90 v95 aDASTIDAR19923 a
Gamma-irradiated splenic homogenates of armadillos infected with M. leprae proved sterile by conventional tests and media. However, on media for chemoautotrophy, these could repeatedly grow as a single type of acid-fast nocardioform bacterium like the unirradiated specimens, although with a much reduced count. In the slide culture, transition from the initial AFB/coccoid bodies, to sporulating mycelia and granules in the final stage, could be observed sequentially. The gamma-irradiated tissue specimens failed to yield any other mycobacterium/corynebacterium tested according to standard protocols.
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