01226nas a2200265   4500000000100000008004100001260001300042653001200055653002400067653001900091653004000110653001300150653001100163653002400174653002800198653002000226100001900246700001500265700001400280245013600294300001000430490000700440520049900447022001400946       1992                            d  c1992 Aug10aAnimals10aAntigens, Protozoan10aConcanavalin A10aElectrophoresis, Polyacrylamide Gel10aEpitopes10aHumans10aLeishmania donovani10aLeishmaniasis, Visceral10aSerologic Tests1 aBhattacharya M1 aBanerjee N1 aGhosh D K00aLeishmania donovani: isolation of a concanavalin-A specific antigen and its evaluation for serodiagnosis of visceral leishmaniasis.  a341-50 v863 a<p>A glycoconjugate antigen of 27-39 kDa was isolated from a cell-free extract of Leishmania donovani by affinity chromatography using a Concanavalin-A sepharose-4B column and eluted with 0.5 M alpha-methylmannoside. The antigen was recognized specifically by sera from kala-azar (visceral leishmaniasis) patients and did not react with sera from tuberculosis, leprosy or malaria patients. The antigen may therefore be useful in developing a serodiagnostic assay for visceral leishmaniasis.</p>  a0003-4983