01843nas a2200397   4500000000100000008004100001260001300042653001200055653002600067653002400093653001900117653001700136653002000153653001300173653001600186653002400202653001100226653002100237653002400258653001200282653000900294653002500303653002200328653001200350653002400362100001400386700001400400700001600414700001300430700001300443245012900456300001100585490000600596520082900602022001401431       1992                            d  c1992 Dec10aAnimals10aAntibodies, Bacterial10aAntigens, Bacterial10aAutoantibodies10aAutoantigens10aCross Reactions10aEpitopes10aGuinea Pigs10aHeat-Shock Proteins10aHumans10aImmunoglobulin G10aIn Vitro Techniques10aleprosy10aMice10aMycobacterium leprae10aPeripheral nerves10aRabbits10aSpecies Specificity1 aLaunois P1 aN'Diaye M1 aSarthou J L1 aMillan J1 aBach M A00aAnti-peripheral nerve antibodies in leprosy patients recognize an epitope shared by the M. leprae 65 kDa heat shock protein.  a745-570 v53 a<p>Binding of leprosy sera to peripheral nerve from different species (mouse, guinea pig and rabbit) was evaluated by ELISA. A majority of sera, whatever the clinical form of leprosy, bind to these antigens. Absorption with Mycobacterium bovis BCG demonstrated that these antibodies recognize cross-reactive epitopes between peripheral nerve and mycobacteria. In immunoblot analysis, both leprosy patient sera and a monoclonal antibody directed at the 65 kDa heat shock protein of M. leprae were shown to react with a heat-shock 67-68 kDa sciatic nerve protein. Binding of the monoclonal antibody to this sciatic nerve antigen was prevented by incubation with lepromatous patient sera, showing that some peripheral nerve epitopes recognized by patient antibodies are shared by the 65 kDa heat shock protein of M. leprae.</p>  a0896-8411