01890nas a2200289   4500000000100000008004100001260001600042653001500058653002000073653002700093653001500120653001100135653001200146653001600158653000900174653003800183653004300221653003000264100001400294700001500308700001200323245011600335300001000451490000800461520111700469022001401586       1992                            d  c1992 Dec 1610aBenzamides10aCells, Cultured10aChromosome Aberrations10aDNA Repair10aHumans10aleprosy10aLymphocytes10aMale10aPoly Adenosine Diphosphate Ribose10aPoly(ADP-ribose) Polymerase Inhibitors10aSister Chromatid Exchange1 aD'Souza D1 aThomas I M1 aDas B C00aEffect of inhibitor of poly (ADP-ribose) polymerase in blood lymphocyte cultures of untreated leprosy patients.  a251-50 v2843 a<p>Poly (ADP-ribose) polymerase is a cellular repair enzyme synthesised following damage to DNA. 3-Aminobenzamide (3-AB) is an inhibitor of this repair enzyme. To study repair efficiency in leprosy patients, who usually show a significantly higher frequency of spontaneous chromosome aberrations and sister-chromatid exchanges (SCEs), their blood lymphocyte cultures were treated with 3-AB. A marginal increase in the frequency of chromosome aberrations was observed following treatment with 3-AB in controls as well as in patient groups. There was also no significant difference in the frequency of SCEs in control cultures with or without 3-AB. A significant increase in the frequency of SCEs was observed in lymphocyte cultures of paucibacillary (PB) and multibacillary (MB) patients treated with 3-AB when compared with controls. Observation of a significant increase in the frequency of SCEs in 3-AB-treated cultures over the untreated value indicates that DNA damage caused in leprosy patients following mycobacterial infection is not repaired because of the presence of the inhibitor of repair enzyme.</p>  a0027-5107