01605nas a2200313   4500000000100000008004100001260001600042653001900058653004100077653001100118653001000129653002300139653001200162653002500174653001900199653003000218653002600248653004900274653002700323100001000350700001000360700001100370700001100381245013400392300001000526490000700536520073400543022001401277       2003                            d  c2003 May 1510aDNA, Bacterial10aDrug Resistance, Multiple, Bacterial10aHumans10aKorea10aLeprostatic Agents10aleprosy10aMycobacterium leprae10aPoint Mutation10apolymerase chain reaction10aPolymorphism, Genetic10aPolymorphism, Single-Stranded Conformational10aSequence Analysis, DNA1 aKim S1 aLee S1 aKang T1 aChae G00aDetection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR.  a27-320 v363 a<p>The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively.</p>  a0928-8244